The TROVO system by Enrich Biosystems is a high-throughput, microfluidics-free platform designed to establish potent CAR-T cell clones for strong tumor therapies, together with mind cancers. By using 3D-printed hydrogel microwells, TROVO facilitates the co-culture of CAR-T cells with tumor cells, enabling real-time imaging and evaluation of T cell-tumor interactions. This setup permits for the evaluation of key practical metrics resembling cytotoxicity and proliferation of effector cells. Superior AI-driven software program processes this knowledge to pick out and retrieve high-performing T cell clones for downstream functions.
On this seminar, we demonstrated the power to isolate tumor-reactive T cells with out reliance on predefined biomarkers, streamlining the invention of efficient clones for CAR-T improvement. Moreover, we showcased how this platform aids in uncovering distinct cytotoxicity-relevant pathways in 3D versus 2D cultures. This methodology enhances the effectivity and scalability of creating CAR-T cell therapies for strong tumors.
Highlights:
- Perceive the problem of CAR-T clone choice for strong tumors and its important implications for therapeutic efficacy.
- Uncover a streamlined methodology to evaluate T cell performance utilizing the TROVO system’s 3D co-culture workflow with out counting on predefined biomarkers.
- See how AI-driven evaluation of real-time cytotoxicity knowledge permits dependable identification of high-performing clones, making it a scalable method for CAR-T improvement.
